This technical brief provides guidance to countries on how they can strategically invest the Global Fund resources in HIV prevention programming for adolescent girls and young women (AGYW) in the 2023-2025 allocation cycle as part of their national response to HIV. This brief replaces previous guidance issued in 2019 and reflects the most recent guidance received from UN agencies and partner organizations. It should be used in conjunction with other key Global Fund documents providing guidance on preparation of funding requests for the 2023-2025 allocation period.